1. Create a list. (from the main menu, select 'List' followed by 'New'...)
2. From the main menu, select 'Parse' followed by 'Several sequences'.
3. Select a sequence database and range of sequences to be parsed. Click the 'OK' button.
4. The 'Parse Specifications' dialog box appears. Use the default output file (since you probably won't look at it anyway), and select the 'list to be update' (use the list just created).
5. Decide if you want to zero the use counts
• if yes, the frequency counts in the list are set to 0.
• if no, the existing frequency counts in the list are used as the starting values. This allows you to use the list to generate a cummulative frequency count of sequences from several different databases.
6. Click 'OK' to start parsing the sequences.
7. After parsing is complete, a dialog box appears allowing you to view/delete/print the output file. Click the 'delete' button (you only need to view this file if you're interested in parsing errors).
8. Repeat steps 3 thru 7 (but do not zero the frequency counts) until all sequences have been processed
9. At this point, the list contains the frequency counts for the sequences.

To print the frequency counts:

• From the main menu, select 'List', then 'Print a list' then '(F4) Frequency count'.

To print the calls NOT used yet:

• From the main menu, select 'List', then 'Print a list' then press F1, F2, F3, or F5 (I suggest F3 as it is the most compact; then set the use count min to 0 and the use count max to 0.

To view the frequency counts:

• From the main menu, select 'List' then 'View a list' then 'Display/L-H side/Use-count' then 'Display/Change use count range' and set minimum to 0 and maximum to 9999.

To view the calls NOT used yet:

• From the main menu, select 'List' then 'View a list' then 'Display/Change use count range' and set minimum to 0 and maximum to 0.